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  1. Related Articles
  2. Pulmonary Alveolar Proteinosis
  3. Can the Assessment of Malaria Severity be Simplified for Non-immune African Children?

Cell 44 , — Poobalasingam, T. Imaging the lung: the old ways and the new. Snoeck, H.

"Respiratory Distress in the Newborn" by Megan Connelly for OPENPediatrics

Modeling human lung development and disease using pluripotent stem cells. Development , 13—16 Hind, M. Alveolar proliferation, retinoid synthesizing enzymes, and endogenous retinoids in the postnatal mouse lung. Different roles for Aldh-1 and Raldh Amy, R. Postnatal growth of the mouse lung. Pieretti, A. A novel in vitro model to study alveologenesis. Chen, H. Effect of hyperoxia on retinoid metabolism and retinoid receptor expression in the lungs of newborn mice. Love, D. Frank, D. Emergence of a wave of Wnt signaling that regulates lung alveologenesis by controlling epithelial self-renewal and differentiation.

Cell Rep. Sanderson, M. Exploring lung physiology in health and disease with lung slices. Meng, Q. Cell Sci. Thornton, E. Live imaging of the lung. Chapter 12 , Unit Burgstaller, G. Multidimensional immunolabeling and 4D time-lapse imaging of vital ex vivo lung tissue. Lung Cell. Murray, J. Methods for imaging thick specimens: confocal microscopy, deconvolution, and structured illumination.

Cold Spring Harb. Messier, E. Efficient and rapid isolation and purification of mouse alveolar type II epithelial cells. Lung Res. Hasegawa, K. Fujino, N. A novel method for isolating individual cellular components from the adult human distal lung.


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Lichtman, J. Fluorescence microscopy. Methods 2 , — Biggs, D. Chapter 12 , 11—20 Unit 12 Icy: an open bioimage informatics platform for extended reproducible research. Methods 9 , — Kepiro, M. Norman, L.

Blebbing dynamics during endothelial cell spreading. Cell Biol.

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Schliwa, M. Action of cytochalasin D on cytoskeletal networks. Londhe, V. Hyperoxia impairs alveolar formation and induces senescence through decreased histone deacetylase activity and up-regulation of p21 in neonatal mouse lung. Mankouski, A.

Related Articles

Intermittent hypoxia during recovery from neonatal hyperoxic lung injury causes long-term impairment of alveolar development: A new rat model of BPD. Gomes, A.

Oxygen partial pressure is a rate-limiting parameter for cell proliferation in 3D spheroids grown in physioxic culture condition. Schindelin, J. Fiji: an open-source platform for biological-image analysis. Chenouard, N. Multiple hypothesis tracking for cluttered biological image sequences. Pattern Anal. Download references. Conceived the study: C. All authors contributed to editing the manuscript.

Correspondence to Charlotte H. Journal peer review information : Nature Communications thanks the anonymous reviewers for their contribution to the peer review of this work. Peer reviewer reports are available. Reprints and Permissions. By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Advanced search.

Pulmonary Alveolar Proteinosis

Skip to main content. Subjects Cellular imaging Development. Abstract Damage to alveoli, the gas-exchanging region of the lungs, is a component of many chronic and acute lung diseases. Introduction The primary function of the lungs is gas exchange and the site for this is the alveoli 1 , 2. Full size image. Discussion We have established a method for visualising murine alveologenesis in real time. Methods Mice All animal maintenance and procedures were carried out according to the requirements of the Animal Scientific Procedures Act Precision-cut lung slicing and in vitro culture Precision-cut lung slices PCLS were obtained from mouse lungs according to the protocol described in ref.

Cell tracking and proliferation analysis using Icy software Icy version 1. Image deconvolution and movie generation Z-stacks, either single; static or multiple; time-lapse, were acquired by widefield microscopy. Data availability The data that support the findings of this study are available either in the manuscript or supplementary files.

Can the Assessment of Malaria Severity be Simplified for Non-immune African Children?

References 1. Article Google Scholar 4. Article Google Scholar 6. Article Google Scholar Google Scholar Article Google Scholar Download references. Ethics declarations Competing interests The authors declare no competing interests. Additional information Journal peer review information : Nature Communications thanks the anonymous reviewers for their contribution to the peer review of this work. Supplementary information Supplementary Information. Peer Review File. Description of Additional Supplementary Files. Supplementary Movie 1a-b. Supplementary Movie 2a-b. Supplementary Movie 3a-b.

Supplementary Movie 4a-b. Supplementary Movie5a. Supplementary Movie 5b.


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